RayBiotech manufactures over 1,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare urine samples for ELISA Kits.
Preparing urine samples for ELISA Kits
- Collect urine without adding stabilizers.
- Centrifuge the samples hard (eg. 10,000 x g for 1 min or 5,000 x g for 2 min).
- Aliquot, quick freeze in dry ice/methanol bath, and store at -80°C until use.
Note: Regardless of the sample type you have, it is strongly recommended that you sub-aliquot all samples after preparation to minimize protein degradation from multiple freeze-thaw cycles. This also ensures availability of sample for further experiments.
For more information about our ELISA Kits and sample preparation, visit www.raybiotech.com.