ELISA is an essential analytical technique in many labs, with broad applications in molecular diagnostics, medicine, plant pathology, and basic life science research. Due to the exquisitely high sensitivity and specificity of ELISA, it is widely considered to be the “gold standard” for macromolecule quantification in biological fluids.
Maximizing ELISA’s accuracy and sensitivity is highly dependent on the composition, handling, and storage of the sample being tested. Unlike Western blot, ELISA is conducted under non-denaturing conditions, detecting proteins directly in solution in their native state. Because ELISA can accommodate a wide variety of aqueous biological matrices, the researcher may encounter many potential pitfalls when acquiring specimens of an unfamiliar type. How should fresh biopsy tissue be extracted to maintain the native state of the target protein? How should body fluids be prepared and stored to prevent matrix effects in the assay? Guidelines for sample preparation and storage will be discussed here for the sample types most commonly used with ELISA.
Since 2001, RayBiotech has established a leading reputation for high quality and innovation in immunoassay development. Our ELISA kit line has expanded to include over 1000 fully validated, GMP-compliant ELISA kits for efficient analysis of proteins involved in inflammation, angiogenesis, apoptosis, cell growth, and signal transduction. Every ELISA kit we manufacture is backed by our 90-day 100% satisfaction guarantee so you can evaluate our kits with no risks, no worries. Learn More!