7 Ways to Study Protein Phosphorylation

7 Ways to Study Protein Phosphorylation

Introduction Protein phosphorylation is the most well-studied post translational modification (PTM), in which a phosphoryl group from adenosine triphosphate (ATP) is covalently attached to a serine (~86%), threonine (~12%), or tyrosine (~2%) by a kinase and removed by a phosphatase (Figure 1). [1] Phosphorylation at other amino acids have also…

Rapid ELISA-Based Measurement of Protein Phosphorylation

Immunoblotting is frequently used for immunodetection of phosphoproteins, in some cases following immunoprecipitation of the cellular extract. The signals from each probe can then be compared and phospho-specific fractions visually assessed or quantified by densitometry. However, the time and effort required in these protocols has driven many researchers to seek…