A Comparison of Antibody Arrays and Mass Spectrometry in Protein Profiling and Biomarker Research

A Comparison of Antibody Arrays and Mass Spectrometry in Protein Profiling and Biomarker Research

Highlights Antibody arrays: removal of high abundance proteins is not required, but antibody availability limits protein detection. Mass spectrometry: “hypothesis-free” protein detection is affected by high abundance proteins and peptide ionization. Both high throughput technologies are capable of semi-quantitative and quantitative data. Antibody arrays and mass spectrometry complement each other.…