High-Density Screening Arrays Can Help You Focus Your Research Efforts

High-Density Screening Arrays Can Help You Focus Your Research Efforts

Autophagy has been shown to play a critical role in kidney maintenance, disease, and aging. In this study, the authors identified a novel mechanism by which IFN-γ activates autophagy in human kidney epithelial cells. They found that IFN-γ promoted tryptophan depletion, activated the eIF2α kinase general control nonderepressible-2 (GCN2), and…

Assessing Protein Panels Can Indicate How They Work Within Networks To Control Complex Processes

Assessing Protein Panels Can Indicate How They Work Within Networks To Control Complex Processes

The aim of this study was to characterize cellular processes that are altered in mammary endothelial cells (MECs) displaying reduced cyclin D1 activity in an effort to better understand the biological effects of pharmacological approaches to treat breast cancer. The use of an antibody array was instrumental in determining that…

Antibody Arrays Can Provide Insight Into Processes Regulating Cell Function

Antibody Arrays Can Provide Insight Into Processes Regulating Cell Function

Telomere shortening leads to the induction of DNA damage signaling, which has been associated with aging and stem cell decline. In this study, the authors used a cytokine array to help determine that telomere dysfunction impaired mesenchymal progenitor cell function, reduced the capacity of bone marrow stromal cells to maintain…

High-Content Screens Are Essential To Identify Secreted Factors Relevant To Biological Processes

High-Content Screens Are Essential To Identify Secreted Factors Relevant To Biological Processes

The authors of this study identify DNA damage signaling as a key mediator of vascular smooth muscle cell (VSMC) osteogenic differentiation in a model of calcification and aging. They demonstrate that the accumulation of prelamin A, a nuclear lamin protein that plays an important role in VSMC calcification, interferes with…

Cytokine Arrays Can Help Advance Your Research

Cytokine Arrays Can Help Advance Your Research

In this study the authors investigated induction of senescence in human melanoma as a potential cancer therapeutic approach. They found that inhibition of aurora kinase induced senescence which resulted from the DNA damage response. The use of an antibody array indicated that this DNA damage mediated IKKβ/NF-κB activation and promoted…

Shedding Light into the Tumor Microenvironment

Shedding Light into the Tumor Microenvironment

Tumors are a heterogeneous group of cells from diverse organs, ranging from stem cells and endothelial cells, to a wide range of immune cells. The plethora of secretory signals from cancer cells have numerous effects that help promote tumor growth and progression, while also perturbing the immunologic surveillance of developing…