Recombinant Proteins in Research

Recombinant Proteins in Research

Introduction Proteins play an integral role in human health. As such, basic, applied, and clinical research aim to understand how protein expression, localization, post translational modifications (PTMs), and interactions regulate homeostasis and disease (see yellow box). Our current understanding of how proteins affect cellular phenotype is based on numerous applications,…

A Comparison of Antibody Arrays and Mass Spectrometry in Protein Profiling and Biomarker Research

A Comparison of Antibody Arrays and Mass Spectrometry in Protein Profiling and Biomarker Research

Highlights Antibody arrays: removal of high abundance proteins is not required, but antibody availability limits protein detection. Mass spectrometry: “hypothesis-free” protein detection is affected by high abundance proteins and peptide ionization. Both high throughput technologies are capable of semi-quantitative and quantitative data. Antibody arrays and mass spectrometry complement each other.…

Exosomes: A Source of Protein Biomarkers

Exosomes are 30-150 nm extracellular vesicles that are endocytically released from cells. Exosomes were previously thought to simply exude unnecessary proteins from cells; however, more recent research has highlighted their importance in cell-to-cell communication by transporting biomolecules such as mRNA, microRNA, and protein through the circulatory system. Recipient cells may…