Preparing Conditioned Media Samples for ELISA Kits

ELISA Kit RayBiotech manufactures over 1,000 high quality, rigorously tested ELISA kits. In this blog post we explain how to prepare conditioned media samples for your ELISA Kit. How to Prepare Conditioned Media Samples We recommend preparing serum-free or low-serum medium samples, as serum tends to contain cytokines which may…

Preparing Plasma and Serum Samples for ELISA Kits

RayBiotech manufactures over 1,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare plasma and serum samples for ELISA Kits. How to Prepare plasma and serum samples for ELISA Kits For plasma: Collect whole blood into an EDTA, Citrate or Sodium heparin tube (e.g.…

Preparing Urine Samples for ELISA Kits

RayBiotech manufactures over 1,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare urine samples for ELISA Kits. Preparing urine samples for ELISA Kits Collect urine without adding stabilizers. Centrifuge the samples hard (eg. 10,000 x g for 1 min or 5,000 x g…

Preparing cell or tissue lysates for ELISA Kits

RayBiotech manufactures over 2,000 high fully validated, GMP-compliant ELISA kits. In this blog post we explain how to prepare cell or tissue lysates for ELISA Kits. Preparing cell or tissue lysates for ELISA Kits Cell or tissue lysates for use with RayBio® ELISA kits can be prepared using most conventional…

Rapid ELISA-Based Measurement of Protein Phosphorylation

Immunoblotting is frequently used for immunodetection of phosphoproteins, in some cases following immunoprecipitation of the cellular extract. The signals from each probe can then be compared and phospho-specific fractions visually assessed or quantified by densitometry. However, the time and effort required in these protocols has driven many researchers to seek…

Finding Improved Markers of Acute Kidney Injury

In the January 2014 Issue of Clinical Laboratory News, published by the AACC (American Association for Clinical Chemistry), one of the cover stories was The Search for Improved Markers of Acute Kidney Injury. The article’s main points are that: The current markers used to assess acute kidney injury (AKI), creatinine…

High-Density Screening Arrays Can Help You Focus Your Research Efforts

High-Density Screening Arrays Can Help You Focus Your Research Efforts

Autophagy has been shown to play a critical role in kidney maintenance, disease, and aging. In this study, the authors identified a novel mechanism by which IFN-γ activates autophagy in human kidney epithelial cells. They found that IFN-γ promoted tryptophan depletion, activated the eIF2α kinase general control nonderepressible-2 (GCN2), and…

Assessing Protein Panels Can Indicate How They Work Within Networks To Control Complex Processes

Assessing Protein Panels Can Indicate How They Work Within Networks To Control Complex Processes

The aim of this study was to characterize cellular processes that are altered in mammary endothelial cells (MECs) displaying reduced cyclin D1 activity in an effort to better understand the biological effects of pharmacological approaches to treat breast cancer. The use of an antibody array was instrumental in determining that…

Antibody Arrays Can Provide Insight Into Processes Regulating Cell Function

Antibody Arrays Can Provide Insight Into Processes Regulating Cell Function

Telomere shortening leads to the induction of DNA damage signaling, which has been associated with aging and stem cell decline. In this study, the authors used a cytokine array to help determine that telomere dysfunction impaired mesenchymal progenitor cell function, reduced the capacity of bone marrow stromal cells to maintain…

High-Content Screens Are Essential To Identify Secreted Factors Relevant To Biological Processes

High-Content Screens Are Essential To Identify Secreted Factors Relevant To Biological Processes

The authors of this study identify DNA damage signaling as a key mediator of vascular smooth muscle cell (VSMC) osteogenic differentiation in a model of calcification and aging. They demonstrate that the accumulation of prelamin A, a nuclear lamin protein that plays an important role in VSMC calcification, interferes with…